Portions of the following information were taken from Florida Geological Survey Bulletin 66.

The Holton Creek Rise is 11 miles northwest of Live Oak on SRWMD land. From the intersection of US 90 and CR 249 in Live Oak, drive northwest on CR 249 (Noble’s Ferry Road) approximately 12 miles to the bridge over the Suwannee River. From the bridge travel approximately 0.3 mile to the second right graded road. Follow SRWMD signs to Holton Creek. The spring is at the head of the creek.

Description - The spring pool measures 225 ft northwest to southeast and 177 ft northeast to southwest. Along the north shore, a vertical limestone ledge drops quickly off to a depth of 100 ft; however, the bottom is highly irregular in the rest of the depression. The water is dark and tannic (water was reported as clear in Rosenau et al., 1977). There is very little aquatic and emergent vegetation in the spring pool. No boil was observed during October 2001. The spring has steep sandy banks that rise to approximately 25 ft above water level, and the high ground is forested with pines and oaks. Holton Creek Rise discharges through Holton Creek, a run that meanders generally southeast approximately 1 mile to the Suwannee River.

Utilization - Land around the spring is pristine and owned by the SRWMD. The Florida National Scenic Trail winds along the north side of Holton Creek.