Portions of the following information were taken from Florida Geological Survey Bulletin 66.

Treehouse Spring is approximately 2 miles (3.2 km) north of High Springs on the east bank of the Santa Fe River. The spring can be accessed by boat from a public boat ramp downstream from the spring. From the junction of US 441/41 and CR 236 (Main Street) in High Springs, drive north on US 441/41 approximately 1.2 miles (1.9 km). Turn west (left) at public access boat sign just before the Santa Fe River. The spring is 0.6 miles (1 km) upstream from the boat ramp on the southeast side of the river.

Description - Treehouse Spring is in a circular cove on the southeast side of the Santa Fe River. The spring discharges westward into the adjacent river. Spring pool diameter measures 125 ft (38.1 m) north to south and 175 ft (53.3 m) east to west. Pool depth over the vent is 31 ft (9.4 m). Water color was tannic, and there was no spring boil during October 2001. Water hyacinth was the only non-native plant species observed in the spring pool. No other vegetation could be seen through the dark water. Land adjacent to this spring is a forested lowland flood plain. The nearest high ground is approximately 150 ft (46 m) to the east, and it rises 10-12 ft (3-3.7 m) higher than the flood plain and is forested with mixed hardwoods and pines. Treehouse Spring is also published as ALA112971 (Hornsby and Ceryak, 1998).

Utilization -the land surrounding this spring is privately owned and is pristine. There is a small rope swing on the east side and the spring is a local swimming spot.